Kit0204 manual

Upon thawing, total RNA was extracted using the PicoPure kit (KIT, Thermo Fisher) and External RNA Controls Consortium (ERCC) spike-ins added at 1: 10^5 dilution. cDNA was synthesized and amplified using the Ovation RNA-seq v2 kit (NuGEN), yielding µg of cDNA, and sheared using a Covaris E sonicator. The Arcturus® PicoPure® RNA Isolation Kit is designed to recover high-quality total RNA consistently from fewer than ten cells, even from a single cell. This kit is designed to provide consistent, efficient RNA recovery without sacrificing quality. Small volume elution allows you to maximize your recovery of RNA from small numbers of cells.  · RNA Isolation from Pico-scale samples (1 cell (pg) - 10mg tissue) with the PicoPure. Dissolve the solid DNase I ( Kunitz units) in μl RNase-free water. Divide the stock solution into single-use aliquots, and store oC for up to 9 months. Thawed aliquots can be stored at oC for up to 6 weeks. Do not refreeze aliquots after www.doorway.rug: manual.

RNA Isolation from Pico-scale samples with the PicoPure ™ • Protocol for Use with CapSure™ Macro LCM Caps • Protocol for Use with <10mg Frozen Tissue Columns can handle: 1 cell (pg) – 10mg tissue. The Arcturus® PicoPure® RNA Isolation Kit is designed to recover high-quality total RNA from fewer than 10 cells―even from a single cell. This kit is designed to provide consistent, efficient RNA recovery without sacrificing quality. Low-volume elution allows you to maximize RNA recovery from small numbers of cells for gene expression analysis. RNA Isolation from Pico-scale samples (1 cell (pg) - 10mg tissue) with the PicoPure. Dissolve the solid DNase I ( Kunitz units) in μl RNase-free water. Divide the stock solution into single-use aliquots, and store oC for up to 9 months. Thawed aliquots can be stored at oC for up to 6 weeks. Do not refreeze aliquots after thawing.

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