Hplc manual injector troubleshooting

 · Posted by: Mubarak Patel. Hi guys, My name is Mubarak Patel and I’m a Master of Pharmacy with experience in Pharmaceutical Manufacturing Industries. Here are some good SOPs used in Pharmaceutical formulations. Also you can get here GMP, cGMP, ICH guidelines, specification of products etc. Post a Comment. Appendix C and Appendix D discuss artifacts mistaken as injector problems. Ensure vent tubes are properly leveled (see Symptom 2) to prevent contamination from siphoning into the loop. To flush the needle port: Use to 1 mL of mobile phase. Use only a Needle Port Cleaner (IDEX Health Science Part Number ) attached to a luer tip syringe. 4 www.doorway.ru Problems with Ghost Peaks Possible Cause Prevention/Solution Ghost Peaks Contamination in column or injector 1. Use only HPLC grade solvents 2. Flush column to remove impurities (see ) 3. Flush injector between analyses Late eluting peak from previous injection 1. Extend run time (see ) 2. Flush column with strong mobile phaseFile Size: KB.

Isolating HPLC Problems. In an HPLC system, problems can arise from many sources. First. problems are due to blockages in the system or guard col. •Remove Column - Pressure Still High? •Remove Guard – Pressure Still High? •If Column pressure is high: • Back flush column – Clear “dirty” frit surface • Wash column – Eliminate column contamination and plugged packing – high molecular weight/adsorbed compounds. INJECTOR. z. DETECTOR. z. DATA COLLECTION. z. BAND SPREADING/ CONNECTIONS. z. COLUMNS. z. SAMPLE VIALS. Troubleshooting Strategy. Try to simplify --assess impact on lab efficiency --inspect the chromatography --try to categorize. troubleshoot the easiest to fix items first. MECHANICAL.

Japan Analytical Industry Recycling Preparative HPLC LC-NEXT series Manual Injector Replacement Procedure. Rheodyne Manual Sample Injector Valves These HPLC sample injectors can inject from 1µL to 5mL with high troubleshoot and does not dilute sample. The relative reproducibility of peak areas for isocratic HPLC analyses should be at least 1% or Bypass the autoinjector and install a manual injector.